Western blot. All lanes: RGS14 antibody at 3.88ug/ml. Lane 1: 293T whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: SH-SY5Y whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 62, 22, 45, 37 kDa. Observed band size: 62 kDa.