Western blot. All lanes: RAE1 antibody at 4.39ug/ml. Lane 1: Mouse brain tissue. Lane 2: HepG2 whole cell lysate. Lane 3: 293T whole cell lysate. Lane 4: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 41 kDa. Observed band size: 41, 80 kDa.