Western blot. All lanes: Ras-related protein Rab-5A antibody at 5ug/ml. Lane 1: Mouse brain tissue. Lane 2: HL60 whole cell lysate. Lane 3: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 24, 23 kDa. Observed band size: 24 kDa.