Western Blot. Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, Rat kidney tissue. All lanes: PTPN12 antibody at 7.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 89, 73, 75 kDa. Observed band size: 89 kDa