Western blot. All lanes: PRPS1antibody at 6.28ug/ml. Lane 1: HepG2 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 35, 28 kDa. Observed band size: 35 kDa.