Western blot. All lanes: PRDX5 antibody at 4µg/ml. Lane 1: Mouse brain tissue. Lane 2: Hela whole cell lysate. Lane 3: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 23, 18, 13 kDa. Observed band size: 23 kDa