Western blot. All lanes: PPIB antibody at 9µg/ml. Lane 1: NIH/3T3 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 24 kDa. Observed band size: 24 kDa