Western blot. All lanes: PPEF1 antibody at 1µg/ml. Lane 1: U251 whole cell lysate. Lane 2: A549 whole cell lysate. Lane 3: Mouse gonadal tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 76, 70, 73, 45, 69 kDa. Observed band size: 76 kDa