Western blot. All lanes: PDHA2 antibody at 1.62 ug/ml. Lane 1: Mouse gonadal tissue. Lane 2: 293T whole cell lysate. Lane 3: A549 whole cell lysate. Lane 4: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 43 kDa. Observed band size: 43 kDa.