Western blot. All lanes: BUD31 antibody at 5µg/ml. Lane 1: Jurkat whole cell lysate. Lane 2: Raji whole cell lysate. Lane 3: NIH/3T3 whole cell lysate. Lane 4: K562 whole cell lysate. Lane 5: HepG2 whole cell lysate. Lane 6: U251 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 17, 18 kDa. Observed band size: 17 kDa