Western blot. All lanes: Paired box protein Pax-4 antibody at 6ug/ml. Lane 1: Hela whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: Mouse pancreatic. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 38, 30 kDa. Observed band size: 38 kDa.