Western blot. All lanes: OTX2 antibody at 4µg/ml. Lane 1: Jurkat whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: Mouse lung tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 32, 33 kDa. Observed band size: 32 kDa