Western blot. All lanes: Nuclear receptor subfamily 2 group C member 1 antibody at 10ug/ml. Lane 1: PC-3 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 68, 52, 54 kDa. Observed band size: 68 kDa.