Western blot. All lanes: NOX3antibody at 0.96ug/ml. Lane 1: Mouse kidney tissue. Lane 2: 293T whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 65 kDa. Observed band size: 65 kDa.