Western blot. All lanes: NCL antibody at 4ug/ml. Lane 1: Jurkat whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 77 kDa. Observed band size: 77 kDa.