Western blot. All lanes: MTHFD2 antibody at 4.99µg/ml. Lane 1: 293 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: Hela whole cell lysate. Lane 5: K562 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 38, 27 kDa. Observed band size: 38 kDa