Western blot. All lanes: DNA replication licensing factor MCM3 antibody at 8ug/ml. Lane 1: Hela whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 91, 96 kDa. Observed band size: 91 kDa.