Western blot. All lanes: Loxl2 antibody at 14µg/ml. Lane 1: 293T whole cell lysate. Lane 2: A549 whole cell lysate. Lane 3: A431 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 88, 82 kDa. Observed band size: 88 kDa