Western blot. All lanes: Kit ligand antibody at 4ug/ml. Lane 1: HepG2 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 31, 28, 27 kDa. Observed band size: 31 kDa.