Western blot. All lanes: IL2RG antibody at 12µg/ml. Lane 1: Jurkat whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: HL60 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 43, 21 kDa. Observed band size: 43 kDa