Western blot. All lanes: IL1B antibody at 2µg/ml. Lane 1: Recombinant Interleukin-1 beta protein 0.05µg. Lane 2: Recombinant Interleukin-1 beta protein 0.5µg. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 31 kDa. Observed band size: 31 kDa