Western blot. All lanes: HUS1 antibody at 2µg/ml. Lane 1: Hela whole cell lysate. Lane 2: 293T whole cell lysate. Lane 2: A431 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 32, 30 kDa. Observed band size: 32 kDa