Western blot. All lanes: Corticosteroid 11-beta-dehydrogenase isozyme 1 antibody at 7ug/ml. Lane 1: Mouse liver tissue. Lane 2: Jurkat whole cell lysate. Lane 3: LO2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 32 kDa. Observed band size: 32 kDa.