Western blot. All lanes: HPD antibody at 4.5µg/ml. Lane 1: Mouse kidney tissue. Lane 2: Mouse liver tissue. Lane 3: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 45, 41 kDa. Observed band size: 45 kDa