Western Blot. Positive WB detected in: K562 whole cell lysate, A549 whole cell lysate (treated with 30mM sodium butyrate for 4h or not). All lanes: HIST1H4A antibody at 1.05µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 12 kDa. Observed band size: 12 kDa