Western Blot. Detected samples: K562 whole cell lysate, A549 whole cell lysate; Untreated (-) or treated (+) with 10mM disodium succinylate for 4h. All lanes: HIST1H4A antibody at 1:100. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 12 kDa. Observed band size: 12 kDa