Western Blot. Detected samples: A549 whole cell lysate; Untreated (-) or treated (+) with 100ng/ml EGF for 20min. All lanes: HIST1H2BC antibody at 1:100. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 14 kDa. Observed band size: 14 kDa