Western Blot. Positive WB detected in: 293 whole cell lysate (treated with 30mM sodium butyrate for 4h). All lanes: HIST1H2BC antibody at 1.27µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 14 kDa. Observed band size: 14 kDa