Western Blot. Positive WB detected in: Rat heart tissue, Rat liver tissue. All lanes: HDAC8 antibody at 2.5ug/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 42, 30, 18, 32, 29, 16, 17 kDa. Observed band size: 42 kDa.