Western blot. All lanes: HAT1 antibody at 3µg/ml. Lane 1: MCF-7 whole cell lysate. Lane 2: 293T whole cell lysate. Lane 3: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit at 1/10000 dilution. Predicted band size: 50, 40 kDa. Observed band size: 50 kDa