Western blot. All lanes: GSTK1 antibody at 2µg/ml. Lane 1: 293T whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: A431 whole cell lysate. Lane 4: COLO205 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 26, 32, 25, 21 kDa. Observed band size: 32 kDa