Western blot. All lanes: Interferon-induced guanylate-binding protein 1 antibody at 4ug/ml. Lane 1: MM231 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: MGF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 68 kDa. Observed band size: 68 kDa.