Western blot. All lanes: Fxn antibody at 2µg/ml. Lane 1: HepG2 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: Mouse brain tissue. Lane 4: Jurkat whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 23 kDa. Observed band size: 23 kDa