Western blot. All lanes: EEF1G antibody at 0.78µg/ml. Lane 1: Mouse gonadal tissue. Lane 2: PC-3 whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: Hela whole cell lysate. Lane 5: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 51, 57 kDa. Observed band size: 51 kDa