Western blot. All lanes: ECHS1 antibody at 3.55µg/ml. Lane 1: Rat heart tissue. Lane 2: Mouse liver tissue. Lane 3: Mouse skeletal muscle tissue. Lane 4: Hela whole cell lysate. Lane 5: HepG2 whole cell lysate. Lane 6: MCF-7 whole cell lysate. Lane 7: Jurkat whole cell lysate. Lane 8: A549 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 32 kDa. Observed band size: 32 kDa