Western blot. All lanes: ECHS1 antibody at 3.03µg/ml. Lane 1: Mouse liver tissue. Lane 2: Mouse skeletal muscle tissue. Lane 3: Hela whole cell lysate. Lane 4: HepG2 whole cell lysate. Lane 5: MCF-7 whole cell lysate. Lane 6: Jurkat whole cell lysate. Lane 7: A549 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 32 kDa. Observed band size: 32 kDa