Western blot. All lanes: DR1 antibody at 2.32ug/ml. Lane 1: Rat gonad tissue. Lane 2: PC-3 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 19 kDa. Observed band size: 23, 26 kDa.