Western blot. All lanes: DHRS3 antibody at 0.8µg/ml. Lane 1: A375 whole cell lysate. Lane 2: Mouse liver tissue. Lane 3: Mouse spleen tissue. Lane 4: HL60 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 34, 19 kDa. Observed band size: 19 kDa