Western blot. All lanes: DGKA antibody at 6.48µg/ml. Lane 1: Hela whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 83, 14, 29 kDa. Observed band size: 83 kDa