Western blot. All lanes: 28S ribosomal protein S29, mitochondrial antibody at 10ug/ml. Lane 1: Hela whole cell lysate. Lane 2: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 46, 42 kDa. Observed band size: 46 kDa.