Western blot. All lanes: CLK1antibody at 3.07ug/ml. Lane 1: HepG2 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: U251 whole cell lysate. Lane 4: A549 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 58, 17, 62 kDa. Observed band size: 58 kDa.