Western blot. All lanes: Chloride intracellular channel protein 1 antibody at 4ug/ml. Lane 1: Hela whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: MCF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 27 kDa. Observed band size: 27 kDa.