Western blot. All lanes: CLGN antibody at 7µg/ml. Lane 1: Mouse brain tissue. Lane 2: Mouse lung tissue. Lane 3: Jurkat whole cell lysate. Lane 4: HepG2 whole cell lysate. Lane 5: PC-3 whole cell lysate. Lane 6: Hela whole cell lysate. Lane 7: HL60 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 71, 47 kDa. Observed band size: 80 kDa