Western blot. All lanes: CHRNG antibody at 5.5ug/ml. Lane 1: 293T whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 58, 53 kDa. Observed band size: 58 kDa.