Western blot. All lanes: Rat Transferrin antibody at 2µg/ml. Lane 1: Rat Transferrin at 0.2 mg. Lane 2: Rat Transferrin at 0.1 mg. Secondary. Goat polyclonal to rabbit IgG at 1/15000 dilution. Predicted band size: 77, 55 kDa. Observed band size: 77 kDa