Western blot. All lanes: CBX5 antibody at 9µg/ml. Lane 1: K562 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: 293T whole cell lysate. Lane 4: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 23 kDa. Observed band size: 23 kDa