Western blot. All lanes: Calpain-1 catalytic subunit antibody at 2ug/ml. Lane 1: A431 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: Jurkat whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 82 kDa. Observed band size: 82 kDa.