Western blot. All lanes: CA3 antibody at 2µg/ml. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 30 kDa. Observed band size: 30 kDa