Western blot. All lanes: EVA1C antibody at 5µg/ml. Lane 1: Hela whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: 293T whole cell lysate. Lane 4: HL60 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 50, 8 kDa. Observed band size: 50 kDa