Western blot. All lanes: ATXN3 antibody at 2µg/ml. Lane 1: 293T whole cell lysate. Lane 2: Mouse brain tissue. Lane 3: MCF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 42, 36, 40, 21 kDa. Observed band size: 42 kDa